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KMID : 0358420100530020160
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Korean Journal of Obstetrics and Gynecology
2010 Volume.53 No. 2 p.160 ~ p.168
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Effects of simvastatin on the proliferation and apoptosis of human endometrial stromal cells from women with endometriosis
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Moon Soo-Hyeon
Lee Seong-Eui
Kim Hwi-Gon
Choi Ook-Hwan
Lee Kyu-Sup
Na Yong-Jin
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Abstract
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Objective: To evaluate the effects of simvastatin on the proliferation and apoptosis of human endometrial cells from women with endometriosis.
Methods: Endometrial tissues were obtained from four women with endometriosis. The endometrial stromal cells isolated from tissue were cultured with 0, 2 and 10 μM simvastatin treatments for 48 hours. The proliferation of endometrial stromal cells was inhibited with 2 and 10 μM simvastatin treatments compared to control. The effect of simvastatin on the sub-G1 phase of cell cycle was determined by flow cytometry. The expression of apoptosis related molecule (Bcl-2, Bax and caspase-3) was examined in control and simvastatin treatments using western blot.
Results: The sub-G1 phase was higher in 10 μM simvastatin than in control and 2 μM simvastatin (P<0.05). This result showed that simvastatin could induce apoptosis of stromal cells. The expression of Bcl-2 was increased in simvastatin treatments slightly (P<0.05) and the expression of Bax was not different between control and experimental groups. The activation of caspase-3 was significantly higher in 10 μM simvastatin treated group than control and 2 μM simvastatin treated groups (P<0.05).
Conclusion: Simvastatin induces apoptosis of endometrial stromal cells and inhibits their proliferation. It was considered that simvastatin could potentially be a therapeutic agent for the treatment of endometriosis.
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KEYWORD
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Endometriosis, Simvastatin, Endometrial stromal cell, Apoptosis
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